当前位置:  首页 >> 最新重要论文

最新重要论文

Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme, ACS Catal, 23 Apr 2021

发布时间:2021年04月23日

ACS Catalysis, 23 April, 2021, DOI:盛大彩票幸运28

Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme

Fuying Kang, Lu Yu, Yan Xia, Minling Yu, Lin Xia, Yuchuan Wang, Lin Yang, Tianyuan Wang, Weimin Gong, Changlin Tian*, Xiaohong Liu*, and Jiangyun Wang*

Abstract

Photosystem I (PSI) is a very large membrane protein complex (~1000 kDa) harboring P700*, the strongest reductant known in biological systems, which is responsible for driving NAD(P)+ and ultimately for CO2 reduction. Although PSI is one of the most important components in the photosynthesis machinery, it has remained difficult to enhance PSI functions through genetic engineering due to its enormous complexity. Inspired by PSI’s ability to undergo multiple-step photo-induced electron hopping from P700* to iron–sulfur [Fe4S4] clusters, we designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (FeA/FeB). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to FeA/FeB, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process.

文章链接:https://pubs.acs.org/doi/full/10.1021/acscatal.1c00287

最新报道:http://sdcpxy28.766bbin.com/kyjz/zxdt/202104/t20210425_5996822.html

 

 

    附件下载:
网站地图 百合彩票北京时时彩 盛大彩票湖北快3 盛大彩票幸运28
现金网开户送彩金 菲律宾申博娱乐官方网 申博sunbet msc 菲律宾太阳城
对战牛牛下载 澳门现金赌场 吉祥彩票加拿大28 菲律宾申博太阳城主页登入
百合彩票腾讯分分彩 盛大彩票安徽快三 盛大彩票加拿大28 百合彩票黑龙江时时彩
盛大彩票排列三、五 百合彩票加拿大3.5分 百合彩票河南快3 盛大彩票北京PK拾
986tt.com 233PT.COM 778DC.COM 1112978.COM XSB598.COM
189sunbet.com S618B.COM 2222ib.com 292SUN.COM TONGSHISHI.COM
178sunbet.com 178sunbet.com 298PT.COM 98csb.com 555TGP.COM
8ZJS.COM 127sun.com 157psb.com 999sbsg.com 505sj.com